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Structure and function predictions of hypothetical proteins in Vibrio Phages

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Author(s): Swapnil G. Sanmukh | Waman N. Paunika | Tarun K. Ghosh | Tapan Chakrabarti

Journal: International Journal of Biometric and Bioinformatics
ISSN 1985-2347

Volume: 4;
Issue: 5;
Start page: 161;
Date: 2010;
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Keywords: Bioinformatics Web Tools | Conserved Domains | Protein Structure Prediction | Uncharacterized Proteins | Life Cycle and Pathogenicity

ABSTRACT
The Vibriophages are the potential agents for the transfer of the virulence factor to their host through lateral gene transfer. The complete genome sequencing of various known vibriophages has been done which deciphered the presence of various gene sequences for hypothetical proteins whose function is not yet understood. We analyzed complete genome of 21such Vibriophages for hypothetical proteins from which 13 phages were sorted for our studies. Our attempt is to predict the structure and function of these hypothetical proteins by the application of computational methods and Bioinformatics. The probable function prediction of the hypothetical protein was done by using Bioinformatics web tools like CDD8BLAST, INTERPROSCAN, PFAM and COGs by searching sequence databases for the presence of orthologous enzymatic conserved domains in the hypothetical sequences. While tertiary structures were constructed using PS2 Server (Protein Structure Prediction server). These study revealed presences of enzymatic functional domain in 92 uncharacterized proteins; their roles are yet to be discovered in Vibriophages. These deciphered enzymatic data for hypothetical proteins can be used for the understanding of functional, structural, evolutionary and metabolic development of Vibriophages and its life cycle along with their role in host evolution and pathogenicity