Noncovalent Labeling of Biomolecules with Red and Near- Infrared DyesAuthor(s): Gabor Patonay | Jozef Salon | John Sowell | Lucjan Strekowski
Journal: Molecules ISSN 1420-3049
Volume: 9; Issue: 3; Start page: 40; Date: 2004;
Keywords: Cyanine dyes | squarylium dyes | protein | DNA | capillary electrophoresis | fluorescence.
Biopolymers such as proteins and nucleic acids can be labeled with a fluorescent marker to allow for their detection. Covalent labeling is achieved by the reaction of an appropriately functionalized dye marker with a reactive group on a biomolecule. The recent trend, however, is the use of noncovalent labeling that results from strong hydrophobic and/or ionic interactions between the marker and biomolecule of interest. The main advantage of noncovalent labeling is that it affects the functional activity of the biomolecule to a lesser extent. The applications of luminescent cyanine and squarylium dyes are reviewed.