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Noncovalent Labeling of Biomolecules with Red and Near- Infrared Dyes

Author(s): Gabor Patonay | Jozef Salon | John Sowell | Lucjan Strekowski

Journal: Molecules
ISSN 1420-3049

Volume: 9;
Issue: 3;
Start page: 40;
Date: 2004;
Original page

Keywords: Cyanine dyes | squarylium dyes | protein | DNA | capillary electrophoresis | fluorescence.

Biopolymers such as proteins and nucleic acids can be labeled with a fluorescent marker to allow for their detection. Covalent labeling is achieved by the reaction of an appropriately functionalized dye marker with a reactive group on a biomolecule. The recent trend, however, is the use of noncovalent labeling that results from strong hydrophobic and/or ionic interactions between the marker and biomolecule of interest. The main advantage of noncovalent labeling is that it affects the functional activity of the biomolecule to a lesser extent. The applications of luminescent cyanine and squarylium dyes are reviewed.
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