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Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA

Author(s): Alexandra H. Heussner | Simon Ausländer | Daniel R. Dietrich

Journal: Toxins ISSN 2072-6651
Volume: 2; Issue: 6; Start page: 1582; Date: 2010;
Original page

Keywords: mycotoxin | ochratoxin B | monoclonal antibody | ELISA | western analysis

ABSTRACT
A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 µg/L (8 nM), a limit of quantification (LOQ) of 3.7 µg/L (10 nM), and a 50% inhibitory concentration (IC50) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.