Atomic Force Microscope Imaging of the Aggregation of Mouse Immunoglobulin G MoleculesAuthor(s): Jiye Cai | Yao Chen | Qingcai Xu | Yong Chen | Tao Zhao | Xiaoyan Wang | Ke Xia
Journal: Molecules ISSN 1420-3049
Volume: 8; Issue: 1; Start page: 86; Date: 2003;
Keywords: Mouse immunoglobulin G | self-assembly | aggregation | atomic force microscope
Mouse immunoglobulin G (Ig G1 and the mixture of Ig G1 and Ig G2) deposited on mica were imaged with an atomic force microscope at room temperature and ambient pressure. At a concentration around 1.0mg/L, the molecules were well dispersed. 2~3 days after sample preparation, both Ig G1 and the mixture could self- assemble into different shapes and further form some types of local-ordered toroidal aggregations (monotoroidal, intercrossed toroidal, concentric toroidal, etc.). The number of monomers was not identical in the different toroidal aggregations but in a same circle, the shapes of polymer self-assembled by several monomolecules were found to be almost the same. There was difference between the aggregation behavior of Ig G1 and the mixture. The mechanism of Ig G molecule aggregation was ascribed to the Ã¢Â€ÂœYÃ¢Â€Â shape and loops structure of Ig G molecule.