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Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts

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Author(s): Eleonora Fusi | Raffaella Rebucci | Chiara Pecorini | Anna Campagnoli | Luciano Pinotti | Francesca Saccone | Federica Cheli | Stig Purup | Kristen Sejrsen | Antonella Baldi

Journal: Toxins
ISSN 2072-6651

Volume: 2;
Issue: 6;
Start page: 1265;
Date: 2010;
Original page

Keywords: ochratoxin A | α-tocopherol | DNA damage | fibroblasts | swine

ABSTRACT
The aims of the current study were to determine the half-lethal concentration of ochratoxin A (OTA) as well as the levels of lactate dehydrogenase release and DNA fragmentation induced by OTA in primary porcine fibroblasts, and to examine the role of α-tocopherol in counteracting its toxicity. Cells showed a dose-, time- and origin-dependent (ear vs. embryo) sensitivity to ochratoxin A. Pre-incubation for 3 h with 1 nM α-tocopherol significantly (P < 0.01) reduced OTA cytotoxicity, lactate dehydrogenase release and DNA damage in both fibroblast cultures. These findings indicate that α-tocopherol supplementation may counteract short-term OTA toxicity, supporting its defensive role in the cell membrane.