Polyvinyl alcohol-glutaraldehyde as solid-phase in Elisa for Schistosomiasis
Author(s): ARAíšjO Aureci M., BARBOSA Gustavo H.T.S., DINIZ Josæ Ricardo P., MALAGUEí‘O Elizabeth, AZEVEDO Walter M., CARVALHO Jr. Luiz B. deKeywords: Polyvinyl alcohol, Glutaraldehyde, ELISA, Schistosoma manson
Journal: Revista do Instituto de Medicina Tropical de São Paulo
Year: 1997
Full text: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651997000300006
Publisher: Instituto de Medicina Tropical
Abstract: Soluble adult Schistosoma mansoni antigen preparation (SWAP) was covalently fixed onto polyvinyl alcohol-glutaraldehyde discs and an enzyme linked-immunosorbent assay (ELISA) was set up. The best conditions for the assay were established and it was found that small amount of antigen such as 1.5 µg was required. A comparison between this procedure and the conventional ELISA was proceeded. A reliable method of antigen immobilization was achieved and the low prices of the employed reagents are economically attractive